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Expression and Purification of the Human Cation-chloride Cotransporter KCC1 from HEK293F Cells for Structural Studies
Cation-chloride cotransporters (CCCs) mediate the coupled, electroneutral symport of cations such as Na(+) and/or K(+) with chloride across membrane. Among CCCs family, K-Cl cotransporters (KCC1-KCC4) extrude intracellular Cl- by the transmembrane K(+) gradient. In humans, these KCCs play vital role...
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| Publicado en: | Bio Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Bio-Protocol
2021
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8054176/ https://ncbi.nlm.nih.gov/pubmed/33889660 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3966 |
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