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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substrates and assess enzyme specificity

Deubiquitylating enzymes (DUBs) counteract ubiquitylation to control stability or activity of substrates. Identification of DUB substrates is challenging because multiple DUBs can act on the same substrate, thwarting genetic approaches. Here, we circumvent redundancy by chemically inhibiting multipl...

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Detalles Bibliográficos
Publicado en:Cell Chem Biol
Main Authors: Rossio, Valentina, Paulo, Joao A., Chick, Joel, Brasher, Bradley, Gygi, Steven P., King, Randall W.
Formato: Artigo
Idioma:Inglês
Publicado: 2021
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC8052291/
https://ncbi.nlm.nih.gov/pubmed/33417828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2020.12.007
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