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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substrates and assess enzyme specificity

Deubiquitylating enzymes (DUBs) counteract ubiquitylation to control stability or activity of substrates. Identification of DUB substrates is challenging because multiple DUBs can act on the same substrate, thwarting genetic approaches. Here, we circumvent redundancy by chemically inhibiting multipl...

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Detalhes bibliográficos
Publicado no:Cell Chem Biol
Main Authors: Rossio, Valentina, Paulo, Joao A., Chick, Joel, Brasher, Bradley, Gygi, Steven P., King, Randall W.
Formato: Artigo
Idioma:Inglês
Publicado em: 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC8052291/
https://ncbi.nlm.nih.gov/pubmed/33417828
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2020.12.007
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