A reversed phase HPLC method for the quantification of HIV gp145 glycoprotein levels from cell culture supernatants

A reversed phase high performance liquid chromatography (RP-HPLC) method was developed for the quantitative determination of recombinant HIV-1 gp145 produced in CHO-K1 cells, as measured directly from culture supernatants. Samples were diluted in 50% D-PBS and 50% PowerCHO-2 (PC2) spent medium, and...

Full description

Saved in:
Bibliographic Details
Published in:J Chromatogr B Analyt Technol Biomed Life Sci
Main Authors: González-Feliciano, José A., Capó-Vélez, Coral M., Akamine, Pearl, Delgado-Vélez, Manuel, Almodóvar, Ruth, Rivera, Javier, Pino, Ignacio, Morell, Gloriner, Eichinger, Daniel, Rivera, José H., Lasalde-Dominicci, José A., Baerga-Ortiz, Abel
Format: Artigo
Language:Inglês
Published: 2021
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC8041096/
https://ncbi.nlm.nih.gov/pubmed/33571843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2021.122562
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items