A reversed phase HPLC method for the quantification of HIV gp145 glycoprotein levels from cell culture supernatants

A reversed phase high performance liquid chromatography (RP-HPLC) method was developed for the quantitative determination of recombinant HIV-1 gp145 produced in CHO-K1 cells, as measured directly from culture supernatants. Samples were diluted in 50% D-PBS and 50% PowerCHO-2 (PC2) spent medium, and...

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Publicado en:J Chromatogr B Analyt Technol Biomed Life Sci
Autores principales: González-Feliciano, José A., Capó-Vélez, Coral M., Akamine, Pearl, Delgado-Vélez, Manuel, Almodóvar, Ruth, Rivera, Javier, Pino, Ignacio, Morell, Gloriner, Eichinger, Daniel, Rivera, José H., Lasalde-Dominicci, José A., Baerga-Ortiz, Abel
Formato: Artigo
Lenguaje:Inglês
Publicado: 2021
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC8041096/
https://ncbi.nlm.nih.gov/pubmed/33571843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2021.122562
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