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A reversed phase HPLC method for the quantification of HIV gp145 glycoprotein levels from cell culture supernatants
A reversed phase high performance liquid chromatography (RP-HPLC) method was developed for the quantitative determination of recombinant HIV-1 gp145 produced in CHO-K1 cells, as measured directly from culture supernatants. Samples were diluted in 50% D-PBS and 50% PowerCHO-2 (PC2) spent medium, and...
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| Publicado en: | J Chromatogr B Analyt Technol Biomed Life Sci |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2021
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8041096/ https://ncbi.nlm.nih.gov/pubmed/33571843 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2021.122562 |
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