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Evaluation of carboxyfluorescein-labeled 7-methylguanine nucleotides as probes for studying cap-binding proteins by fluorescence anisotropy

Fluorescence anisotropy (FA) is a powerful technique for the discovery of protein inhibitors in a high-throughput manner. In this study, we sought to develop new universal FA-based assays for the evaluation of compounds targeting mRNA 5′ cap-binding proteins of therapeutic interest, including eukary...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Wojtczak, Anna, Kasprzyk, Renata, Warmiński, Marcin, Ubych, Krystian, Kubacka, Dorota, Sikorski, Pawel J., Jemielity, Jacek, Kowalska, Joanna
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2021
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8032668/
https://ncbi.nlm.nih.gov/pubmed/33833335
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-87306-8
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