Genome Oligopaint via Local Denaturation Fluorescence in Situ Hybridization (GOLD FISH)

Cas9 in complex with a programmable guide RNA targets specific double-stranded DNA for cleavage. By harnessing Cas9 as a programmable loader of superhelicase to genomic DNA, here we report a physiological-temperature DNA FISH method termed Genome Oligopaint via Local Denaturation Fluorescence in Sit...

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Publicat a:Mol Cell
Autors principals: Wang, Yanbo, Cottle, Wayne Taylor, Wang, Haobo, Feng, Xinyu Ashlee, Mallon, John, Gavrilov, Momcilo, Bailey, Scott, Ha, Taekjip
Format: Artigo
Idioma:Inglês
Publicat: 2021
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC8026568/
https://ncbi.nlm.nih.gov/pubmed/33657402
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2021.02.011
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