A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin

Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to id...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Li, Feng, Hung, Stevephen, Esnault, Caroline, Levin, Henry L.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2021
Fag:
Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8024771/
https://ncbi.nlm.nih.gov/pubmed/33855304
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100392
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