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A protocol for transposon insertion sequencing in Schizosaccharomyces pombe to identify factors that maintain heterochromatin
Transposon insertion sequencing (TIS) is a highly effective method used with bacteria to identify genes important for growth in any condition of interest. Previously, we adapted this method to identify essential genes of the yeast Schizosaccharomyces pombe. Here, we describe modifications used to id...
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| Publicado no: | STAR Protoc |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2021
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8024771/ https://ncbi.nlm.nih.gov/pubmed/33855304 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100392 |
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