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A CRISPR/Cas9 eraser strategy for contamination‐free PCR end‐point detection

Polymerase chain reaction (PCR), a central technology for molecular diagnostics, is highly sensitive but susceptible to the risk of false positives caused by aerosol contamination, especially when an end‐point detection mode is applied. Here, we proposed a solution by designing a clustered regularly...

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Sonraí Bibleagrafaíochta
Foilsithe in:Biotechnol Bioeng
Main Authors: Lin, Wei, Tian, Tian, Jiang, Yongzhong, Xiong, Erhu, Zhu, Debin, Zhou, Xiaoming
Formáid: Artigo
Teanga:Inglês
Foilsithe: John Wiley and Sons Inc. 2021
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8013395/
https://ncbi.nlm.nih.gov/pubmed/33615437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.27718
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