Characterize the Interaction of the DNA Helicase PriA with the Stalled DNA Replication Fork Using Atomic Force Microscopy

In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. ssDNA-binding...

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Udgivet i:Bio Protoc
Main Authors: Wang, Yaqing, Sun, Zhiqiang, Bianco, Piero R., Lyubchenko, Yuri L.
Format: Artigo
Sprog:Inglês
Udgivet: Bio-Protocol 2021
Fag:
Methods Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC8005913/
https://ncbi.nlm.nih.gov/pubmed/33796614
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3940
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