From ELISA to Immunosorbent Tandem Mass Spectrometry Proteoform Analysis: The Example of CXCL8/Interleukin-8

With ELISAs one detects the ensemble of immunoreactive molecules in biological samples. For biomolecules undergoing proteolysis for activation, potentiation or inhibition, other techniques are necessary to study biology. Here we develop methodology that combines immunosorbent sample preparation and...

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Bibliographic Details
Published in:Front Immunol
Main Authors: Metzemaekers, Mieke, Abouelasrar Salama, Sara, Vandooren, Jennifer, Mortier, Anneleen, Janssens, Rik, Vandendriessche, Sofie, Ganseman, Eva, Martens, Erik, Gouwy, Mieke, Neerinckx, Barbara, Verschueren, Patrick, De Somer, Lien, Wouters, Carine, Struyf, Sofie, Opdenakker, Ghislain, Van Damme, Jo, Proost, Paul
Format: Artigo
Language:Inglês
Published: Frontiers Media S.A. 2021
Subjects:
Immunology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7991300/
https://ncbi.nlm.nih.gov/pubmed/33777041
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fimmu.2021.644725
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