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Effect of allotypic variation of human IgG1 on the thermal stability of disulfide-linked knobs-into-holes mutants of the Fc for stable bispecific antibody design

BACKGROUND: Disulfide-linked knobs-into-holes (dKiH) mutation is a well-validated antibody engineering technique to force heterodimer formation of different Fcs for efficient production of bispecific antibodies. An artificial disulfide bond is created between mutated cysteine residues in CH3 domain...

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Detalhes bibliográficos
Publicado no:Antib Ther
Main Authors: Akiba, Hiroki, Satoh, Reiko, Nagata, Satoshi, Tsumoto, Kouhei
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7990158/
https://ncbi.nlm.nih.gov/pubmed/33928224
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/abt/tbz008
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