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Construction and quantitation of a selectable protein splicing sensor using Gibson assembly and spot titers

Inteins (intervening proteins) are translated within host proteins and removed through protein splicing. Conditional protein splicing (CPS), where the rate and accuracy of splicing is highly dependent on environmental cues, has emerged as a novel form of post-translational regulation. While CPS has...

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Dettagli Bibliografici
Pubblicato in:Curr Protoc
Autori principali: Woods, Daniel, LeSassier, Danielle S., Egbunam, Ikechukwu, Lennon, Christopher W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2021
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7988948/
https://ncbi.nlm.nih.gov/pubmed/33739627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.82
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