Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells

The analysis of B cell receptors (BCR) from single B cells is crucial to understanding humoral immune responses. Here, we describe a protocol for the sequencing, cloning, and characterization of antibody genes that encode BCRs. We used this method to analyze the BCRs of different mouse B cell popula...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Viant, Charlotte, Escolano, Amelia, Chen, Spencer T., Nussenzweig, Michel C.
Format: Artigo
Language:Inglês
Published: Elsevier 2021
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7985702/
https://ncbi.nlm.nih.gov/pubmed/33778783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100389
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