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Protocol for high-throughput compound screening using flow cytometry in THP-1 cells

Flow cytometry is a valuable method for analyzing protein expressions at the single cell level but can be difficult to apply to large numbers of samples. This protocol provides instructions to perform a high-throughput small molecule screen using flow cytometry analysis of THP-1 cells, a human monoc...

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Publicat a:STAR Protoc
Autors principals: Spangenberg, Stephan H., Zavareh, Reza Beheshti, Lairson, Luke L.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7985391/
https://ncbi.nlm.nih.gov/pubmed/33778785
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100400
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