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Nuclei isolation of multiple brain cell types for omics interrogation.

We present a nuclei isolation protocol for genomic and epigenomic interrogation of multiple cell type populations in the human and rodent brain. The nuclei isolation protocol allows cell type-specific profiling of neurons, microglia, oligodendrocytes and astrocytes, which is compatible with fresh an...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Nott, Alexi, Schlachetzki, Johannes C. M., Fixsen, Bethany R., Glass, Christopher K.
Format: Artigo
Sprog:Inglês
Udgivet: 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7969463/
https://ncbi.nlm.nih.gov/pubmed/33495627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-020-00472-3
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