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ISOLATION AND FRACTIONATION OF RAT BRAIN NUCLEI

A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate...

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Detalles Bibliográficos
Main Authors: Løvtrup-Rein, H., McEwen, B. S.
Formato: Artigo
Idioma:Inglês
Publicado: The Rockefeller University Press 1966
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107004/
https://ncbi.nlm.nih.gov/pubmed/5968977
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