Engineered FnCas12a with enhanced activity through directional evolution in human cells

Clustered regularly interspaced short palindromic repeat–Cas12a has been harnessed to manipulate the human genome; however, low cleavage efficiency and stringent protospacer adjacent motif hinder the use of Cas12a-based therapy and applications. Here, we have described a directional evolving and scr...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:J Biol Chem
Main Authors: Liu, Xiexie, Liu, Xiaoyu, Zhou, Chenchen, Lv, Jineng, He, Xiubin, Liu, Yuanyuan, Xie, Haihua, Wang, Bang, Lv, Xiujuan, Tang, Lianchao, Li, Mingchun, Liu, Changbao, Zhao, Junzhao, Liu, Yong, Song, Zongming, Gu, Feng
Format: Artigo
Jezik:Inglês
Izdano: American Society for Biochemistry and Molecular Biology 2021
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7961096/
https://ncbi.nlm.nih.gov/pubmed/33567342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbc.2021.100394
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