Development of a colorimetric α-ketoglutarate detection assay for prolyl hydroxylase domain (PHD) proteins

Since the discovery of the prolyl hydroxylases domain (PHD) proteins and their canonical hypoxia-inducible factor (HIF) substrate two decades ago, a number of in vitro hydroxylation (IVH) assays for PHD activity have been developed to measure the PHD–HIF interaction. However, most of these assays ei...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Wong, Samantha J., Ringel, Alison E., Yuan, William, Paulo, Joao A., Yoon, Haejin, Currie, Mark A., Haigis, Marcia C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2021
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7961094/
https://ncbi.nlm.nih.gov/pubmed/33571527
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbc.2021.100397
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