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Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids

Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-as...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Volke, Daniel C., Wirth, Nicolas T., Nikel, Pablo I.
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2021
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7952922/
https://ncbi.nlm.nih.gov/pubmed/33732804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3917
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