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Rapid Genome Engineering of Pseudomonas Assisted by Fluorescent Markers and Tractable Curing of Plasmids

Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-as...

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Vydáno v:Bio Protoc
Hlavní autoři: Volke, Daniel C., Wirth, Nicolas T., Nikel, Pablo I.
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7952922/
https://ncbi.nlm.nih.gov/pubmed/33732804
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3917
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