Comparative CRISPR type III-based knockdown of essential genes in hyperthermophilic Sulfolobales and the evasion of lethal gene silencing

CRISPR type III systems, which are abundantly found in archaea, recognize and degrade RNA in their specific response to invading nucleic acids. Therefore, these systems can be harnessed for gene knockdown technologies even in hyperthermophilic archaea to study essential genes. We show here the broad...

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Detalles Bibliográficos
Publicado en:RNA Biol
Main Authors: Zink, Isabelle Anna, Fouqueau, Thomas, Tarrason Risa, Gabriel, Werner, Finn, Baum, Buzz, Bläsi, Udo, Schleper, Christa
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 2020
Assuntos:
Research Paper
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7951960/
https://ncbi.nlm.nih.gov/pubmed/32957821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2020.1813411
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