Carboxylate-Modified Magnetic Bead (CMMB)-Based Isopropanol Gradient Peptide Fractionation (CIF) Enables Rapid and Robust Off-Line Peptide Mixture Fractionation in Bottom-Up Proteomics

Deep proteome coverage in bottom-up proteomics requires peptide-level fractionation to simplify the complex peptide mixture before analysis by tandem mass spectrometry. By decreasing the number of coeluting precursor peptide ions, fractionation effectively reduces the complexity of the sample leadin...

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Bibliographic Details
Published in:Mol Cell Proteomics
Main Authors: Deng, Weixian, Sha, Jihui, Plath, Kathrin, Wohlschlegel, James A.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2021
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Research
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7950212/
https://ncbi.nlm.nih.gov/pubmed/33476790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.RA120.002411
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