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Systematic analysis of factors that improve homologous direct repair (HDR) efficiency in CRISPR/Cas9 technique

The CRISPR/Cas9 bacterial system has proven to be an powerful tool for genetic manipulation in several organisms, but the efficiency of sequence replacement by homologous direct repair (HDR) is substantially lower than random indel creation. Many studies focused on improving HDR efficiency using dou...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Di Stazio, Mariateresa, Foschi, Nicola, Athanasakis, Emmanouil, Gasparini, Paolo, d’Adamo, Adamo Pio
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7935300/
https://ncbi.nlm.nih.gov/pubmed/33667229
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0247603
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