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Mapping proteolytic neo-N termini at the surface of living cells

N terminomics is a powerful strategy for profiling proteolytic neo-N termini, but its application to cell surface proteolysis has been limited by the low relative abundance of plasma membrane proteins. Here we apply plasma membrane-targeted subtiligase variants (subtiligase-TM) to efficiently and sp...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Weeks, Amy M., Byrnes, James R., Lui, Irene, Wells, James A.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2021
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7923363/
https://ncbi.nlm.nih.gov/pubmed/33536314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2018809118
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