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Cryo-EM grid optimization for membrane proteins
Cryo-EM grid preparation is an important bottleneck in protein structure determination, especially for membrane proteins, typically requiring screening of a large number of conditions. We systematically investigated the effects of buffer components, blotting conditions and grid types on the outcome...
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| Udgivet i: | iScience |
|---|---|
| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Elsevier
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7900225/ https://ncbi.nlm.nih.gov/pubmed/33665558 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2021.102139 |
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