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Cryo-EM grid optimization for membrane proteins

Cryo-EM grid preparation is an important bottleneck in protein structure determination, especially for membrane proteins, typically requiring screening of a large number of conditions. We systematically investigated the effects of buffer components, blotting conditions and grid types on the outcome...

詳細記述

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書誌詳細
出版年:iScience
主要な著者: Kampjut, Domen, Steiner, Julia, Sazanov, Leonid A.
フォーマット: Artigo
言語:Inglês
出版事項: Elsevier 2021
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7900225/
https://ncbi.nlm.nih.gov/pubmed/33665558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2021.102139
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