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A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin

Binding properties of actin-binding proteins are typically evaluated by cosedimentation assays. However, this method is time-consuming, involves multiple steps, and has a limited throughput. These shortcomings preclude its use in screening for drugs that modulate actin-binding proteins relevant to h...

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Detalhes bibliográficos
Publicado no:J Gen Physiol
Main Authors: Bunch, Thomas A., Lepak, Victoria C., Bortz, Kellan M., Colson, Brett A.
Formato: Artigo
Idioma:Inglês
Publicado em: Rockefeller University Press 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7898471/
https://ncbi.nlm.nih.gov/pubmed/33600558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.202012707
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