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Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples

Single mRNA molecules are frequently detected by single molecule fluorescence in situ hybridization (smFISH) using branched DNA technology. While providing strong and background-reduced signals, the method is inefficient in detecting mRNAs within dense structures, in monitoring mRNA compactness and...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Kramer, Susanne, Meyer-Natus, Elisabeth, Stigloher, Christian, Thoma, Hanna, Schnaufer, Achim, Engstler, Markus
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7897490/
https://ncbi.nlm.nih.gov/pubmed/33275141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1142
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