Parallel monitoring of RNA abundance, localization and compactness with correlative single molecule FISH on LR White embedded samples

Single mRNA molecules are frequently detected by single molecule fluorescence in situ hybridization (smFISH) using branched DNA technology. While providing strong and background-reduced signals, the method is inefficient in detecting mRNAs within dense structures, in monitoring mRNA compactness and...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Kramer, Susanne, Meyer-Natus, Elisabeth, Stigloher, Christian, Thoma, Hanna, Schnaufer, Achim, Engstler, Markus
Format: Artigo
Language:Inglês
Published: Oxford University Press 2020
Subjects:
Methods Online
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7897490/
https://ncbi.nlm.nih.gov/pubmed/33275141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1142
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items