PrimeDesign software for rapid and simplified design of prime editing guide RNAs

Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and command-line tool for the design of PE experiment...

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Pubblicato in:Nat Commun
Autori principali: Hsu, Jonathan Y., Grünewald, Julian, Szalay, Regan, Shih, Justine, Anzalone, Andrew V., Lam, Kin Chung, Shen, Max W., Petri, Karl, Liu, David R., Keith Joung, J., Pinello, Luca
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2021
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7884779/
https://ncbi.nlm.nih.gov/pubmed/33589617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-021-21337-7
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