Production of Class II MHC Proteins in Lentiviral Vector-Transduced HEK-293T Cells for Tetramer Staining Reagents

Class II major histocompatibility complex peptide (MHC-IIp) multimers are precisely engineered reagents used to detect T cells specific for antigens from pathogens, tumors, and self-proteins. While the related class I MHC/peptide (MHC-Ip) multimers are usually produced from subunits expressed in E....

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Udgivet i:Curr Protoc
Main Authors: Willis, Richard A., Ramachandiran, Vasanthi, Shires, John C., Bai, Ge, Jeter, Kelly, Bell, Donielle L., Han, Lixia, Kazarian, Tamara, Ugwu, Kyla C., Laur, Oskar, Contreras-Alcantara, Susana, Long, Dale L., Altman, John D.
Format: Artigo
Sprog:Inglês
Udgivet: 2021
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7880703/
https://ncbi.nlm.nih.gov/pubmed/33539685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpz1.36
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