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Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release
In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as...
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| Publicado en: | STAR Protoc |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7876630/ https://ncbi.nlm.nih.gov/pubmed/33615276 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100333 |
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