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Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as...

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Publicat a:STAR Protoc
Autors principals: Perner, Caroline, Sokol, Caroline L.
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7876630/
https://ncbi.nlm.nih.gov/pubmed/33615276
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100333
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