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Biochemical detection of capsid in the nucleus during HIV-1 infection

To understand the role of the HIV-1 capsid in viral replication, we developed a protocol to biochemically track capsid in the nucleus during infection. To this end, we separated HIV-1-infected cells into nuclear and cytosolic fractions. Fractions were analyzed by western blotting for HIV-1 capsid co...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Selyutina, Anastasia, Diaz-Griffero, Felipe
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2021
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7873705/
https://ncbi.nlm.nih.gov/pubmed/33604582
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100323
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