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Metagenomic Quantification of Genes with Internal Standards

We demonstrate that an assembly-independent and spike-in facilitated metagenomic quantification approach can be used to screen and quantify over 2,000 genes simultaneously, while delivering absolute gene concentrations comparable to those for quantitative PCR (qPCR). DNA extracted from dairy manure...

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Publicat a:mBio
Autors principals: Crossette, Emily, Gumm, Jordan, Langenfeld, Kathryn, Raskin, Lutgarde, Duhaime, Melissa, Wigginton, Krista
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2021
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7858063/
https://ncbi.nlm.nih.gov/pubmed/33531401
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.03173-20
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