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Metagenomic Quantification of Genes with Internal Standards
We demonstrate that an assembly-independent and spike-in facilitated metagenomic quantification approach can be used to screen and quantify over 2,000 genes simultaneously, while delivering absolute gene concentrations comparable to those for quantitative PCR (qPCR). DNA extracted from dairy manure...
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| Udgivet i: | mBio |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7858063/ https://ncbi.nlm.nih.gov/pubmed/33531401 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.03173-20 |
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