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Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy

Here we demonstrate that the antigen binding function of a humanized anti-cocaine mAb (h2E2) can be directly and easily determined using simple and inexpensive absorption spectroscopy and dyes commonly used for differential scanning fluorimetry, such as DASPMI and SYPRO Orange. Therapeutic monoclona...

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Bibliografski detalji
Izdano u:Biochem Biophys Res Commun
Glavni autori: Kirley, Terence L., Norman, Andrew B.
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7855642/
https://ncbi.nlm.nih.gov/pubmed/33348081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2020.12.034
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