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Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot

Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at these tRNAs with the modified base. tRNA Q-modifica...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Autores principales: Matuszek, Zaneta, Pan, Tao
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-Protocol 2019
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7854232/
https://ncbi.nlm.nih.gov/pubmed/33654991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3191
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