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Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot
Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at these tRNAs with the modified base. tRNA Q-modifica...
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| Udgivet i: | Bio Protoc |
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| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Bio-Protocol
2019
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7854232/ https://ncbi.nlm.nih.gov/pubmed/33654991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3191 |
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