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Isolation and Culture of Single Myofiber and Immunostaining of Satellite Cells from Adult C57BL/6J Mice

Myofiber isolation followed with ex vivo culture could recapitulate and visualize satellite cells (SCs) activation, proliferation, and differentiation. This approach could be taken to understand the physiology of satellite cells and the molecular mechanism of regulatory factors, in terms of the invo...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Chen, Shujie, Ding, Hongrong, Yao, Xiangping, Xie, Liwei
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2019
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7854163/
https://ncbi.nlm.nih.gov/pubmed/33654822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3313
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