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In vitro RNA Cleavage Assays to Characterize IRE1-dependent RNA Decay
The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage and analysis of RNA substrates of IRE1. The method consists of the in vitro...
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| Publicado no: | Bio Protoc |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7854161/ https://ncbi.nlm.nih.gov/pubmed/33654817 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3307 |
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