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Isolation and Imaging of His- and RFP-tagged Amyloid-like Proteins from Caenorhabditis elegans by TEM and SIM

In our recently published paper, we highlight that during normal aging of C. elegans age-dependent aggregates of proteins form and lead to functional decline of tissues. The protocol described here details the isolation of two proteins from C. elegans in their aggregated amyloid-like form, casein ki...

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Vydáno v:Bio Protoc
Hlavní autoři: Stephens, Amberley D., Lu, Meng, Schierle, Gabriele S. Kaminski
Médium: Artigo
Jazyk:Inglês
Vydáno: Bio-Protocol 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7853938/
https://ncbi.nlm.nih.gov/pubmed/33654909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3408
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