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Genome Engineering Primary Human B Cells Using CRISPR/Cas9.

B cells are lymphocytes derived from hematopoietic stem cells and are a key component of the humoral arm of the adaptive immune system. They make attractive candidates for cell-based therapies because of their ease of isolation from peripheral blood, their ability to expand in vitro, and their longe...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Loaharawee, Kanut, Johnson, Matthew J., Lahr, Walker S., Peterson, Joseph S., Webber, Beau R., Moriarity, Branden S.
Formato: Artigo
Idioma:Inglês
Publicado: 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7851834/
https://ncbi.nlm.nih.gov/pubmed/33226023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/61855
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