In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing

Reprogramming complex cellular metabolism requires simultaneous regulation of multigene expression. Ex-situ cloning-based methods are commonly used, but the target gene number and combinatorial library size are severely limited by cloning and transformation efficiencies. In-situ methods such as mult...

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Pubblicato in:Nat Commun
Autori principali: Wang, Yu, Cheng, Haijiao, Liu, Yang, Liu, Ye, Wen, Xiao, Zhang, Kun, Ni, Xiaomeng, Gao, Ning, Fan, Liwen, Zhang, Zhihui, Liu, Jiao, Chen, Jiuzhou, Wang, Lixian, Guo, Yanmei, Zheng, Ping, Wang, Meng, Sun, Jibin, Ma, Yanhe
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2021
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7846839/
https://ncbi.nlm.nih.gov/pubmed/33514753
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-021-21003-y
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