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Preparation of Drosophila Polytene Chromosomes, Followed by Immunofluorescence Analysis of Chromatin Structure by Multi-fluorescence Correlations

Drosophila larval salivary gland polytene chromosome squashes have been used for decades to analyze genome-wide protein-binding patterns, transcriptional activation processes, and changes in chromatin structure at specific genetic loci. There have been many evolutions of the squashing protocol over...

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Bibliographic Details
Published in:Bio Protoc
Main Authors: Kuhn, Terra M., Little, Shawn C., Capelson, Maya
Format: Artigo
Language:Inglês
Published: Bio-Protocol 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842822/
https://ncbi.nlm.nih.gov/pubmed/33659343
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3673
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