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Measurement of ATPase Activity of Valosin-containing Protein/p97

Valosin-containing protein (VCP; also known as p97) is a type II ATPase regulating several cellular processes. Using proteomic techniques, we identified a chemical compound that binds to the D1 ATPase domain of VCP. The protocol described here was to study the effect of the compound on ATPase activi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Suvarna, Kruthi, Honda, Kaori, Muroi, Makoto, Kondoh, Yasumitsu, Osada, Hiroyuki, Watanabe, Nobumoto
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842740/
https://ncbi.nlm.nih.gov/pubmed/33654741
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3516
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