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Estimating Cellular Abundances of Halo-tagged Proteins in Live Mammalian Cells by Flow Cytometry

Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or require sophisticated experimental and computationa...

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Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Cattoglio, Claudia, Darzacq, Xavier, Tjian, Robert, Hansen, Anders S.
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842713/
https://ncbi.nlm.nih.gov/pubmed/33654751
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3527
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