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Assessing Gα(q/15)-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay

Cell-based functional assays are an important part of compound screening and drug lead optimization, and they can also play a crucial role in the determination of the residues involved in ligand binding and signaling for a particular G-protein-coupled receptor. Conventional methods used for Gα(q/15)...

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Enregistré dans:
Détails bibliographiques
Publié dans:Bio Protoc
Auteurs principaux: Besserer-Offroy, Élie, Brouillette, Rebecca L, Longpré, Jean-Michel, Sarret, Philippe
Format: Artigo
Langue:Inglês
Publié: Bio-Protocol 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842692/
https://ncbi.nlm.nih.gov/pubmed/33659379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3715
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