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Quantification of Protein Kinase A (PKA) Activity by an in vitro Radioactive Assay Using the Mouse Sperm Derived Enzyme

In order to acquire fertilizing potential, mammalian sperm must undergo a process known as capacitation, which relies on the early activation of Protein Kinase A (PKA). Frequently, PKA activity is assessed in whole-cell experiments by analyzing the phosphorylation status of its substrates in a weste...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Stival, Cintia, Graf, Carolina Baro, Visconti, Pablo E., Krapf, Dario
Format: Artigo
Jezik:Inglês
Izdano: Bio-Protocol 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842637/
https://ncbi.nlm.nih.gov/pubmed/33659328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3658
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