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Unbiased and Tailored CRISPR/Cas gRNA Libraries by SynthesizingCovalently-closed-circular (3Cs) DNA

Simplicity, efficiency and versatility of the CRISPR/Cas system greatly contributed to its rapid use in a broad range of fields. Applications of unbiased CRISPR/Cas screenings are increasing and thus there is a growing need for unbiased and tailored CRISPR/Cas gRNA libraries. Conventional methods fo...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Wegner, Martin, Husnjak, Koraljka, Kaulich, Manuel
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842546/
https://ncbi.nlm.nih.gov/pubmed/33654707
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3472
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